ang 1 7 receptor  (MedChemExpress)

Journal: Experimental physiology

Article Title: Centrally administered angiotensin-(1–7) increases the survival of stroke prone spontaneously hypertensive rats

doi: 10.1113/expphysiol.2013.075242

Figure Lengend Snippet: Immediately following weaning at 35 days of age, spSHR were fed a 4% sodium diet. At 49 days of age, rats were infused ICV with either aCSF (0.15 μL/h), Ang-(1–7) [1.0 μg in 0.15 μl aCSF/h] or Ang-(1–7) + A779 (2.5 μg in 0.15 μl aCSF/h) for the next 6 weeks. Animals were monitored and maintained on the 4% sodium diet until death. Following death, brains were removed and the numbers and severity of hemorrhages in the cortex and subcortex/striatum were analyzed as described in the Methods. Panel (A) contains survival curves for spSHR following the above treatments. Vertical black dashed line indicates when the osmotic pumps stopped (91 days of age). N=9 rats for the aCSF and Ang-(1–7) groups; N=6 for the Ang-(1–7) + A779 group. *, P=0.0092, aCSF vs. Ang-(1–7) treatment; P=0.29, aCSF vs. Ang-(1–7) + A-779 treatment; P=0.07, Ang-(1–7) vs. Ang-(1–7) + A-779 treatment. Panels (B) and (D) show the numbers, and panels (C) and (E) the severity of hemorrhages in the cerebral cortex and subcortex/striatum, respectively. Bar graphs are means + SEM. *P<0.05, compared to spSHR infused with aCSF.

Article Snippet: Rabbit anti-Ang-(1–7) Mas receptor antibody was from Alomone Labs (Jerusalem, Israel).

Techniques:

Journal: Experimental physiology

Article Title: Centrally administered angiotensin-(1–7) increases the survival of stroke prone spontaneously hypertensive rats

doi: 10.1113/expphysiol.2013.075242

Figure Lengend Snippet: Panel (A), Sunflower seed eating task: Rats were fed a 4% sodium diet and infused ICV with either aCSF (0.15 μL/h) or Ang-(1–7) [1.0 μg in 0.15 μl aCSF/h] as described in the legend to Figure 1, and underwent behavioral testing via the Sunflower seed-eating task every 3 days from 56 to 102 days of age. Vertical black dashed line indicates when the osmotic pumps stopped (91 days of age). Shown here is the number of shell pieces plotted against age. The timeline shows that the number of remaining shell pieces after eating 5 seeds is reduced by Ang-(1–7) treatment, as indicated by 2 way row matched ANOVA when comparing the lines. Data are means ± SEM. N=6 rats per group. Panels (B) and (C), Immobility and Rotation Frequency: Rats were subjected to the same treatments as in panel (A) and underwent testing for spontaneous locomotor activity between days 105–108 of age as described in the Methods. Bar graphs are means + SEM showing Immobility (B) and Rotation Frequency (C). N=9 rats for the aCSF treatment and 7 for Ang-(1–7). *P<0.05 compared to aCSF-treated spSHR.

Article Snippet: Rabbit anti-Ang-(1–7) Mas receptor antibody was from Alomone Labs (Jerusalem, Israel).

Techniques: Activity Assay

Journal: Experimental physiology

Article Title: Centrally administered angiotensin-(1–7) increases the survival of stroke prone spontaneously hypertensive rats

doi: 10.1113/expphysiol.2013.075242

Figure Lengend Snippet: Rats were fed a 4% sodium diet, infused ICV with either aCSF (0.15 μL/h) or Ang-(1–7) [1.0 μg in 0.15 μl aCSF/h] as described in the legend to Figure 1, and underwent measurements of MAP via tail cuff plethysmography every 2 to 5 days between days 70 and 110 of age. Vertical black dashed line indicates when the osmotic pumps stopped (91 days of age). Data are means ± SEM. N=8 rats per group.

Article Snippet: Rabbit anti-Ang-(1–7) Mas receptor antibody was from Alomone Labs (Jerusalem, Israel).

Techniques:

Journal: Experimental physiology

Article Title: Centrally administered angiotensin-(1–7) increases the survival of stroke prone spontaneously hypertensive rats

doi: 10.1113/expphysiol.2013.075242

Figure Lengend Snippet: (A-F) Rats were fed a 4% sodium diet, infused ICV with either aCSF (0.15 μL/h) or Ang-(1–7) [1.0 μg in 0.15 μl aCSF/h]. (A-F): At 90 days of age rats were deeply anesthetized, perfused transcardially with saline followed by 10% formalin and brains removed for immunostaining as described in the Methods. Panels A and B are representative fluorescence micrographs showing Iba-1 immunoreactivity in the striatum of aCSF and Ang-(1–7) infused rats, respectively. Panels C and D are representative fluorescence micrographs showing NeuN immunoreactivity in the striatum of aCSF and Ang-(1–7) infused rats, respectively. LV= lateral ventricle; cc = Corpus callosum. Stereological analyses of the striatum showing the estimated numbers of Iba-1 positive cells (microglia) and NeuN positive cells (neurons) are shown in panels E and F respectively. The bar graphs are means + SEM. N=5–6 rats/group. *P<0.05 compared to aCSF treated spSHR. (G, H): At 90 days of age, rats were euthanized, brains removed and processed for RT-PCR analyses of MCP-1 (G) and IL-1β (H) respectively. The bar graphs are means + SEM. N=5 rats/group.

Article Snippet: Rabbit anti-Ang-(1–7) Mas receptor antibody was from Alomone Labs (Jerusalem, Israel).

Techniques: Immunostaining, Fluorescence, Reverse Transcription Polymerase Chain Reaction